Large-scale Topographical Screen for Investigation of Physical Neural-Guidance Cues
نویسندگان
چکیده
A combinatorial approach was used to present primary neurons with a large library of topographical features in the form of micropatterned substrate for high-throughput screening of physical neural-guidance cues that can effectively promote different aspects of neuronal development, including axon and dendritic outgrowth. Notably, the neuronal-guidance capability of specific features was automatically identified using a customized image processing software, thus significantly increasing the screening throughput with minimal subjective bias. Our results indicate that the anisotropic topographies promote axonal and in some cases dendritic extension relative to the isotropic topographies, while dendritic branching showed preference to plain substrates over the microscale features. The results from this work can be readily applied towards engineering novel biomaterials with precise surface topography that can serve as guidance conduits for neuro-regenerative applications. This novel topographical screening strategy combined with the automated processing capability can also be used for high-throughput screening of chemical or genetic regulatory factors in primary neurons.
منابع مشابه
A nerve guidance conduit with topographical and biochemical cues: potential application using human neural stem cells
Despite major advances in the pathophysiological understanding of peripheral nerve damage, the treatment of nerve injuries still remains an unmet medical need. Nerve guidance conduits present a promising treatment option by providing a growth-permissive environment that 1) promotes neuronal cell survival and axon growth and 2) directs axonal extension. To this end, we designed an electrospun ne...
متن کاملCompetition between cap and basal actin fiber orientation in cells subjected to contact guidance and cyclic strain
In vivo, adhesive cells continuously respond to a complex range of physical cues coming from the surrounding microenvironment by remodeling their cytoskeleton. Topographical and mechanical cues applied separately have been shown to affect the orientation of the actin stress fibers. Here we investigated the combined effects of contact guidance by topographical cues and uniaxial cyclic strain on ...
متن کاملEffects of Surface Asymmetry on Neuronal Growth
Detailed knowledge of how the surface physical properties, such as mechanics, topography and texture influence axonal outgrowth and guidance is essential for understanding the processes that control neuron development, the formation of functional neuronal connections and nerve regeneration. Here we synthesize asymmetric surfaces with well-controlled topography and texture and perform a systemat...
متن کاملPerceived afterimage size in depth cue-conflict condition
In depth cue-conflict conditions, various depth cues could represent different extents of depth. Previous studies have investigated the perceived size of negative afterimage in depth cue-correlated conditions in which different cues introduce almost the same amounts of depth to the visual system. This study examined the perceived size of the afterimage in the human observers in a condition that...
متن کاملDo visual cues contribute to the neural estimate of viewing distance used by the oculomotor system?
Perceived shape and depth judgments that require knowledge of viewing distance are strongly influenced by both vergence angle and the pattern of vertical disparities across large visual fields. On the basis of this established contribution of visual cues to the neural estimate of viewing distance, we hypothesized that the oculomotor system would also make use of high-level visual cues to distan...
متن کامل